Analytical Methods

Analytical method portfolio

GlycoThera develops, validates and applies state-of-the-art techniques and analytical methods for its client.
The following list provides with an overview about the analytical methods routinely performed at GlycoThera:

Capillary electrophoresis

  • Capillary zone electrophoresis (CZE)
  • Capillary isoelectric focusing (cIEF)
  • Capillary electrophoresis sodium dodecyl sulfate (CE-SDS; reduced and non-reduced)

Circular dichroism

Isoelectric focusing (IEF)

Immuno affinity chromatography techniques

High performance liquid chromatography (HPLC)

  • Reverse Phase (RP)
  • Ion Exchange (IEX)
  • Size Exclusion (SEC)

Mass spectrometry

  • Electrospray Ionisation (ESI)
  • Matrix-Assisted Laser-Desorption/Ionisation (MALDI)
  • MS-MS sequencing of peptides
  • MS-MS sequencing of oligosaccharides

Monoclonal antibody (mAb) purification (and quantification)

  • Protein A
  • Protein G

Peptide mapping (RP-HPLC; optionally with fraction collection)

  • with UV detection
  • and/or MS / MS-MS detection

Quantitative oligosaccharide mapping

  • High-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD)
  • 2-aminobenzamide (2-AB)-labelled glycans + fluorescence detection

Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)

  • one-dimensional (1D)
  • two-dimensional (2D)

UV spectroscopy

Western blotting

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